Journal: European Journal of Medical Research
Article Title: Kinesin-related genes stratified the prognosis and immune responses of clear cell renal cell carcinoma
doi: 10.1186/s40001-025-02880-1
Figure Lengend Snippet: Validation of the role of four signature genes in ccRCC. A – C Overexpression or silencing validation of SLC5A1, PSAT1, and HJURP in ccRCC cells by Western blot. D – F Wound healing results showed that overexpression of SLC5A1 or knockdown of PSAT1 and HJURP inhibited the ccRCC cells migration. G – I Transwell assay showed overexpression of SLC5A1 or knockdown of PSAT1 and HJURP suppressed the ccRCC cells migration and invasion
Article Snippet: Preincubation with 5% goat serum in TBS was performed to minimize non-specific binding, followed by incubation with primary antibodies against SLC5A1 (Cat. ER1916-51, 1:250, HUABIO), WDR72 (Cat. NBP2-31800, 1:1500, NOVUS), PSAT1 (Cat. 10501-1-AP, 1:500, Proteintech), and HJURP (Cat. 15283-1-AP, 1:50, Proteintech).
Techniques: Biomarker Discovery, Over Expression, Western Blot, Knockdown, Migration, Transwell Assay